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Quantitative phase and polarization imaging through an optical fiber applied to detection of early esophageal tumorigenesis.

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Gordon, George SD 
Joseph, James 
Alcolea, Maria P 
Sawyer, Travis 


Phase and polarization of coherent light are highly perturbed by interaction with microstructural changes in premalignant tissue, holding promise for label-free detection of early tumors in endoscopically accessible tissues such as the gastrointestinal tract. Flexible optical multicore fiber (MCF) bundles used in conventional diagnostic endoscopy and endomicroscopy scramble phase and polarization, restricting clinicians instead to low-contrast amplitude-only imaging. We apply a transmission matrix characterization approach to produce full-field en-face images of amplitude, quantitative phase, and resolved polarimetric properties through an MCF. We first demonstrate imaging and quantification of biologically relevant amounts of optical scattering and birefringence in tissue-mimicking phantoms. We present an entropy metric that enables imaging of phase heterogeneity, indicative of disordered tissue microstructure associated with early tumors. Finally, we demonstrate that the spatial distribution of phase and polarization information enables label-free visualization of early tumors in esophageal mouse tissues, which are not identifiable using conventional amplitude-only information.



cancer, optical fibers, polarimetry, quantitative phase imaging, Algorithms, Animals, Esophageal Neoplasms, Esophagus, Female, Image Interpretation, Computer-Assisted, Male, Mice, Mice, Inbred C57BL, Optical Fibers, Optical Imaging, Phantoms, Imaging

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J Biomed Opt

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SPIE-Intl Soc Optical Eng
European Commission (630729)
Cancer Research Uk (None)
Engineering and Physical Sciences Research Council (EP/N014588/1)
Cancer Research UK (C14303/A17197)
Cancer Research UK (21102)
Cancer Research UK (24669)
Engineering and Physical Sciences Research Council (EP/R003599/1)
Cancer Research UK (C609/A17257)
Cancer Research UK (C20/A20976)
Cancer Research UK (A25117)
Medical Research Council (MC_PC_12009)
This work was funded by Cancer Research UK (C47594/A16267, C14303/A17197, C47594/A21102); the European Union Seventh Framework Agreement (FP7-PEOPLE-2013-CIG-630729); and pump priming awards from the Cancer Research UK Cambridge Centre, including dedicated funding from the Early Detection Programme (A20976).